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primary antibodies, ra3-6b2  (Spring Bioscience)

 
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    Spring Bioscience primary antibodies, ra3-6b2
    Primary Antibodies, Ra3 6b2, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies, ra3-6b2/product/Spring Bioscience
    Average 90 stars, based on 1 article reviews
    primary antibodies, ra3-6b2 - by Bioz Stars, 2026-03
    90/100 stars

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    Image Search Results


    a , Confocal microscopy of WT BM section (8μm thick femur) stained with antibodies to IL-7 (red), B220 (blue), Ki67 (yellow), IgM (green) and DAPI (gray) to visualize the location of proliferating and IgM + B cell progenitors. (Single color panels are presented in . The image is representative of 4 independent images from 3 WT mice) b , Distance of IgM + and IgM − Ki67 + B cell progenitors from IL-7 hif niches (white dashed line). Data were pooled from three independent experiments. Distance of each cell counted were shown with mean values (horizontal bars). P values were calculated by unpaired t -test. c , Visualization of B cell progenitors in BM sections (8μm thick femur) of Ck -YFP mice with antibodies to IL-7 (red) and CXCL12 (blue) by confocal microscopy. The image is representative of 3 independent images from 3 WT mice. d , Distances of YFP + B cell progenitors from IL-7 hi niches (white dashed line) in BM of Ck -YFP mice. Data were pooled from two independent experiments. Distance of each cell counted were shown with mean values (horizontal bars). P values were calculated by unpaired t -test. e , Flow cytometric analysis of IL-7 and CXCL12 expression by cultured BM stromal cells (n=3). f, g . Identification of stromal cells expressing IL-7 ( i ) and CXCL12 ( j ) by flow cytometry (n=3). k , Different areas of BM showing varying degrees of IL-7 and CXCL12 expressing stroma by Type 1, Type 2 and Type 3 stromal cells. Images are representative of 5 independent areas from 2 WT BM.

    Journal: Nature immunology

    Article Title: CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis

    doi: 10.1038/s41590-019-0468-0

    Figure Lengend Snippet: a , Confocal microscopy of WT BM section (8μm thick femur) stained with antibodies to IL-7 (red), B220 (blue), Ki67 (yellow), IgM (green) and DAPI (gray) to visualize the location of proliferating and IgM + B cell progenitors. (Single color panels are presented in . The image is representative of 4 independent images from 3 WT mice) b , Distance of IgM + and IgM − Ki67 + B cell progenitors from IL-7 hif niches (white dashed line). Data were pooled from three independent experiments. Distance of each cell counted were shown with mean values (horizontal bars). P values were calculated by unpaired t -test. c , Visualization of B cell progenitors in BM sections (8μm thick femur) of Ck -YFP mice with antibodies to IL-7 (red) and CXCL12 (blue) by confocal microscopy. The image is representative of 3 independent images from 3 WT mice. d , Distances of YFP + B cell progenitors from IL-7 hi niches (white dashed line) in BM of Ck -YFP mice. Data were pooled from two independent experiments. Distance of each cell counted were shown with mean values (horizontal bars). P values were calculated by unpaired t -test. e , Flow cytometric analysis of IL-7 and CXCL12 expression by cultured BM stromal cells (n=3). f, g . Identification of stromal cells expressing IL-7 ( i ) and CXCL12 ( j ) by flow cytometry (n=3). k , Different areas of BM showing varying degrees of IL-7 and CXCL12 expressing stroma by Type 1, Type 2 and Type 3 stromal cells. Images are representative of 5 independent areas from 2 WT BM.

    Article Snippet: For tissue staining, the sections were fixed in 4% paraformaldehyde, blocked with 10% normal donkey serum and stained with primary antibodies to B220 (clone RA3-6B2, eBioscience and ab64100, Abcam), IgM (clone ll/41, eBioscience and FITC-conjugated Goat ant-mouse Jackson 115-097-020/115-096-075), Ki-67 (clone SP6, Ab16667, Lot#GR59808-1;, Abcam), IL-7 (M-19 polyclonal, sc1268, Lot#C2408,Santa Cruz Biotechnology), CXCL12 (FL-93 polyclonal, Santa Cruz Biotechnology and ab18919, Lot#GR116-13, Abcam) in various combinations and thereafter incubated with fluorochrome-conjugated secondary antibodies specific to the primary species and isotypes (Invitrogen).

    Techniques: Confocal Microscopy, Staining, Expressing, Cell Culture, Flow Cytometry

    a , Genomic PCR of WT and floxed alleles for Cxcr4 deletion in tail DNA and flow sorted large pre-B cells from indicated mice with Gapdh as control (n=3). b , Flow cytometric analysis of different developmental stages of B lymphopoiesis in the BM of wild-type (WT), mb1 -cre +/− , Cxcr4 fl/fl and Cxcr4 fl/fl - mb1 -cre +/− littermate control mice (n=9). B cell progenitors are defined as B220 + CD19 + , pro-B cells as B220 + CD19 + CD43 + IgM − , large and small pre-B as B220 + CD19 + CD43 − IgM − FSC hi and B220 + CD19 + CD43 − IgM − FSC lo respectively and immature B cells as B220 + CD19 + CD43 − IgM + . FSC, forward scatter. c , Absolute number of cells per mouse at different stages of B cell development in BM of WT, mb1 -cre +/− , Cxcr4 fl/fl and Cxcr4 fl/fl - mb1 -cre +/− mice (n=9)(Imm B, Immature B cell; Mat B, mature B cells. * P <0.001 compared to all the controls (WT, mb1 -cre +/− , Cxcr4 fl/fl ). Data presented as average ± SD. d,e , Distribution of B cell progenitors in BM of Cxcr4 fl/fl (CXCR4 sufficient; d ) and Cxcr4 fl/fl - mb1 -cre +/− (CXCR4 deficient; e ) mice by confocal microscopy of corresponding BM sections (8μm thick femur) stained with antibodies to IL-7 (red), CXCL12 (blue), B220 (yellow), IgM (green) and DAPI (gray) (n=4). f , Percentage of B cell progenitors (B220 + ) in proximity to IL-7 −/lo CXCL12 + and IL-7 hi CXCL12 +/− stroma (n=4 independent image). Data are presented as mean±SD. Each dot represents average distance obtained from each image of indicated genotype. P values were calculated by unpaired t -test.

    Journal: Nature immunology

    Article Title: CXCR4 signaling directs Igk recombination and the molecular mechanisms of late B lymphopoiesis

    doi: 10.1038/s41590-019-0468-0

    Figure Lengend Snippet: a , Genomic PCR of WT and floxed alleles for Cxcr4 deletion in tail DNA and flow sorted large pre-B cells from indicated mice with Gapdh as control (n=3). b , Flow cytometric analysis of different developmental stages of B lymphopoiesis in the BM of wild-type (WT), mb1 -cre +/− , Cxcr4 fl/fl and Cxcr4 fl/fl - mb1 -cre +/− littermate control mice (n=9). B cell progenitors are defined as B220 + CD19 + , pro-B cells as B220 + CD19 + CD43 + IgM − , large and small pre-B as B220 + CD19 + CD43 − IgM − FSC hi and B220 + CD19 + CD43 − IgM − FSC lo respectively and immature B cells as B220 + CD19 + CD43 − IgM + . FSC, forward scatter. c , Absolute number of cells per mouse at different stages of B cell development in BM of WT, mb1 -cre +/− , Cxcr4 fl/fl and Cxcr4 fl/fl - mb1 -cre +/− mice (n=9)(Imm B, Immature B cell; Mat B, mature B cells. * P <0.001 compared to all the controls (WT, mb1 -cre +/− , Cxcr4 fl/fl ). Data presented as average ± SD. d,e , Distribution of B cell progenitors in BM of Cxcr4 fl/fl (CXCR4 sufficient; d ) and Cxcr4 fl/fl - mb1 -cre +/− (CXCR4 deficient; e ) mice by confocal microscopy of corresponding BM sections (8μm thick femur) stained with antibodies to IL-7 (red), CXCL12 (blue), B220 (yellow), IgM (green) and DAPI (gray) (n=4). f , Percentage of B cell progenitors (B220 + ) in proximity to IL-7 −/lo CXCL12 + and IL-7 hi CXCL12 +/− stroma (n=4 independent image). Data are presented as mean±SD. Each dot represents average distance obtained from each image of indicated genotype. P values were calculated by unpaired t -test.

    Article Snippet: For tissue staining, the sections were fixed in 4% paraformaldehyde, blocked with 10% normal donkey serum and stained with primary antibodies to B220 (clone RA3-6B2, eBioscience and ab64100, Abcam), IgM (clone ll/41, eBioscience and FITC-conjugated Goat ant-mouse Jackson 115-097-020/115-096-075), Ki-67 (clone SP6, Ab16667, Lot#GR59808-1;, Abcam), IL-7 (M-19 polyclonal, sc1268, Lot#C2408,Santa Cruz Biotechnology), CXCL12 (FL-93 polyclonal, Santa Cruz Biotechnology and ab18919, Lot#GR116-13, Abcam) in various combinations and thereafter incubated with fluorochrome-conjugated secondary antibodies specific to the primary species and isotypes (Invitrogen).

    Techniques: Control, Confocal Microscopy, Staining